Immunohistochemistry:Article Title: Adoptive Transfer of Anti-Nucleolin T Cells Combined with PD-L1 Inhibition against Triple-Negative Breast Cancer
Article Snippet: .. The primary antibodies used for IHC, immunofluorescence (IF), Western blotting (WB), and flow cytometry (FC) are listed: Rabbit polyclonal anti-NCL antibody (IHC 1:50; IF 1:50, WB 1:500, FC 1:50, #14574, Cell Signaling Technology, Inc.) or rabbit anti–PD-L1 antibody (IHC 1:50; IF 1:50, WB 1:1,000, FC 1:50, ab205921, Abcam) or rabbit anti-LC3 (IF 1:100; WB 1:2,000, L7543, Sigma-Aldrich Corporation) or mouse anti-SQSTM1/p62 (IF 1:50; WB 1:500, sc-28359, Santa Cruz Biotechnology, Inc.) or mouse anti-BAX (IF 1:100: WB 1:1,000, #610983, Becton Dickinson Holdings PTE. .. Ltd.) or rabbit anti–BCL-2 (WB 1:2,000, ab196495, Abcam) or b-actin (WB 1:10,000, sc-47778, Santa Cruz Biotechnology).
Article Title: Adoptive Transfer of Anti-Nucleolin T Cells Combined with PD-L1 Inhibition against Triple-Negative Breast Cancer
Article Snippet: .. The primary antibodies used for IHC, immunofluorescence (IF), Western blotting (WB), and flow cytometry (FC) are listed: Rabbit polyclonal anti-NCL antibody (IHC 1:50; IF 1:50, WB 1:500, FC 1:50, #14574, Cell Signaling Technology, Inc.) or rabbit anti–PD-L1 antibody (IHC 1:50; IF 1:50, WB 1:1,000, FC 1:50, ab205921, Abcam) or rabbit anti-LC3 (IF 1:100; WB 1:2,000, L7543, Sigma-Aldrich Corporation) or mouse anti-SQSTM1/p62 (IF 1:50; WB 1:500, sc-28359, Santa Cruz Biotechnology, Inc.) or mouse anti-BAX (IF 1:100: WB 1:1,000, #610983, Becton Dickinson Holdings PTE. .. Ltd.) or rabbit anti–BCL-2 (WB 1:2,000, ab196495, Abcam) or β-actin (WB 1:10,000, sc-47778, Santa Cruz Biotechnology).
Immunofluorescence:Article Title: Adoptive Transfer of Anti-Nucleolin T Cells Combined with PD-L1 Inhibition against Triple-Negative Breast Cancer
Article Snippet: .. The primary antibodies used for IHC, immunofluorescence (IF), Western blotting (WB), and flow cytometry (FC) are listed: Rabbit polyclonal anti-NCL antibody (IHC 1:50; IF 1:50, WB 1:500, FC 1:50, #14574, Cell Signaling Technology, Inc.) or rabbit anti–PD-L1 antibody (IHC 1:50; IF 1:50, WB 1:1,000, FC 1:50, ab205921, Abcam) or rabbit anti-LC3 (IF 1:100; WB 1:2,000, L7543, Sigma-Aldrich Corporation) or mouse anti-SQSTM1/p62 (IF 1:50; WB 1:500, sc-28359, Santa Cruz Biotechnology, Inc.) or mouse anti-BAX (IF 1:100: WB 1:1,000, #610983, Becton Dickinson Holdings PTE. .. Ltd.) or rabbit anti–BCL-2 (WB 1:2,000, ab196495, Abcam) or b-actin (WB 1:10,000, sc-47778, Santa Cruz Biotechnology).
Article Title: Adoptive Transfer of Anti-Nucleolin T Cells Combined with PD-L1 Inhibition against Triple-Negative Breast Cancer
Article Snippet: .. The primary antibodies used for IHC, immunofluorescence (IF), Western blotting (WB), and flow cytometry (FC) are listed: Rabbit polyclonal anti-NCL antibody (IHC 1:50; IF 1:50, WB 1:500, FC 1:50, #14574, Cell Signaling Technology, Inc.) or rabbit anti–PD-L1 antibody (IHC 1:50; IF 1:50, WB 1:1,000, FC 1:50, ab205921, Abcam) or rabbit anti-LC3 (IF 1:100; WB 1:2,000, L7543, Sigma-Aldrich Corporation) or mouse anti-SQSTM1/p62 (IF 1:50; WB 1:500, sc-28359, Santa Cruz Biotechnology, Inc.) or mouse anti-BAX (IF 1:100: WB 1:1,000, #610983, Becton Dickinson Holdings PTE. .. Ltd.) or rabbit anti–BCL-2 (WB 1:2,000, ab196495, Abcam) or β-actin (WB 1:10,000, sc-47778, Santa Cruz Biotechnology).
Western Blot:Article Title: Adoptive Transfer of Anti-Nucleolin T Cells Combined with PD-L1 Inhibition against Triple-Negative Breast Cancer
Article Snippet: .. The primary antibodies used for IHC, immunofluorescence (IF), Western blotting (WB), and flow cytometry (FC) are listed: Rabbit polyclonal anti-NCL antibody (IHC 1:50; IF 1:50, WB 1:500, FC 1:50, #14574, Cell Signaling Technology, Inc.) or rabbit anti–PD-L1 antibody (IHC 1:50; IF 1:50, WB 1:1,000, FC 1:50, ab205921, Abcam) or rabbit anti-LC3 (IF 1:100; WB 1:2,000, L7543, Sigma-Aldrich Corporation) or mouse anti-SQSTM1/p62 (IF 1:50; WB 1:500, sc-28359, Santa Cruz Biotechnology, Inc.) or mouse anti-BAX (IF 1:100: WB 1:1,000, #610983, Becton Dickinson Holdings PTE. .. Ltd.) or rabbit anti–BCL-2 (WB 1:2,000, ab196495, Abcam) or b-actin (WB 1:10,000, sc-47778, Santa Cruz Biotechnology).
Article Title: Adoptive Transfer of Anti-Nucleolin T Cells Combined with PD-L1 Inhibition against Triple-Negative Breast Cancer
Article Snippet: .. The primary antibodies used for IHC, immunofluorescence (IF), Western blotting (WB), and flow cytometry (FC) are listed: Rabbit polyclonal anti-NCL antibody (IHC 1:50; IF 1:50, WB 1:500, FC 1:50, #14574, Cell Signaling Technology, Inc.) or rabbit anti–PD-L1 antibody (IHC 1:50; IF 1:50, WB 1:1,000, FC 1:50, ab205921, Abcam) or rabbit anti-LC3 (IF 1:100; WB 1:2,000, L7543, Sigma-Aldrich Corporation) or mouse anti-SQSTM1/p62 (IF 1:50; WB 1:500, sc-28359, Santa Cruz Biotechnology, Inc.) or mouse anti-BAX (IF 1:100: WB 1:1,000, #610983, Becton Dickinson Holdings PTE. .. Ltd.) or rabbit anti–BCL-2 (WB 1:2,000, ab196495, Abcam) or β-actin (WB 1:10,000, sc-47778, Santa Cruz Biotechnology).
Flow Cytometry:Article Title: Adoptive Transfer of Anti-Nucleolin T Cells Combined with PD-L1 Inhibition against Triple-Negative Breast Cancer
Article Snippet: .. The primary antibodies used for IHC, immunofluorescence (IF), Western blotting (WB), and flow cytometry (FC) are listed: Rabbit polyclonal anti-NCL antibody (IHC 1:50; IF 1:50, WB 1:500, FC 1:50, #14574, Cell Signaling Technology, Inc.) or rabbit anti–PD-L1 antibody (IHC 1:50; IF 1:50, WB 1:1,000, FC 1:50, ab205921, Abcam) or rabbit anti-LC3 (IF 1:100; WB 1:2,000, L7543, Sigma-Aldrich Corporation) or mouse anti-SQSTM1/p62 (IF 1:50; WB 1:500, sc-28359, Santa Cruz Biotechnology, Inc.) or mouse anti-BAX (IF 1:100: WB 1:1,000, #610983, Becton Dickinson Holdings PTE. .. Ltd.) or rabbit anti–BCL-2 (WB 1:2,000, ab196495, Abcam) or b-actin (WB 1:10,000, sc-47778, Santa Cruz Biotechnology).
Article Title: Adoptive Transfer of Anti-Nucleolin T Cells Combined with PD-L1 Inhibition against Triple-Negative Breast Cancer
Article Snippet: .. The primary antibodies used for IHC, immunofluorescence (IF), Western blotting (WB), and flow cytometry (FC) are listed: Rabbit polyclonal anti-NCL antibody (IHC 1:50; IF 1:50, WB 1:500, FC 1:50, #14574, Cell Signaling Technology, Inc.) or rabbit anti–PD-L1 antibody (IHC 1:50; IF 1:50, WB 1:1,000, FC 1:50, ab205921, Abcam) or rabbit anti-LC3 (IF 1:100; WB 1:2,000, L7543, Sigma-Aldrich Corporation) or mouse anti-SQSTM1/p62 (IF 1:50; WB 1:500, sc-28359, Santa Cruz Biotechnology, Inc.) or mouse anti-BAX (IF 1:100: WB 1:1,000, #610983, Becton Dickinson Holdings PTE. .. Ltd.) or rabbit anti–BCL-2 (WB 1:2,000, ab196495, Abcam) or β-actin (WB 1:10,000, sc-47778, Santa Cruz Biotechnology).
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